What Is A Microarray Used For?

Question

A microarray is a laboratory tool used to detect the expression of thousands of genes at the same time. DNA microarrays are microscope slides that are printed with thousands of tiny spots in defined positions, with each spot containing a known DNA sequence or gene.

Accepted Answer

A microarray is the recommended first line genetic test for developmental delay (DD), intellectual disability (ID) and autism spectrum disorders (ASD)*. CMA however, does not identify fragile X syndrome (FXS), a common cause of intellectual disability.

Answer

Microarrays can also be used to study the extent to which certain genes are turned on or off in cells and tissues. In this case, instead of isolating DNA from the samples, RNA (which is a transcript of the DNA) is isolated and measured. Today, DNA microarrays are used in clinical diagnostic tests for some diseases.

Answer

Microarray data allows you to determine the level of gene expression in a patient for many genes at one time. In this case, the data provides some insight into which genes may be affected in the development of cancer. 7. In some cancer cells, mRNA is produced (as it would be in a normal cell).

Answer

Blue chips: Microarrays are efficient and accurate at detecting autism variants, but are virtually unknown to most pediatricians and family practice doctors.

Answer

Microarray testing will find common chromosome conditions, like Down syndrome, and can also find chromosome conditions that would not be seen with a karyotype. Microarray results take about two to three weeks.

Answer

A targeted DNA microarray runs from $10 to $100 per sample, says Schena, whereas “the whole human genome is typically $100 [to] $1,000 per sample.” (Targeting strategies are also available for NGS, to avoid the costs incurred by whole-genome sequencing, but they also add both cost and time.)

Answer

In theory, simple inverted sequences are easily detected by CO-FISH, since an inversion will cause the hybridization signal to 'switch' from one sister chromatid to the other.

Answer

Fluorescence in situ hybridization (FISH) can detect specific sites of specific DNA sequences in metaphase or interphase cells. This technique, initially developed for mammalian chromosome, was first applied to plant chromosomes by Schwarzacher et al.

Answer

RNA FISH (RNA fluorescence in situ hybridization) is a powerful technique that enables the visualization and localization of RNA and protein targets in fixed cells.

Answer

The most well known use of DNA microarrays is for profiling messenger RNA levels; however, DNA microarrays have also been used to detect DNA–protein (e.g. transcription factor-binding site and transcription factor) interactions, alternatively spliced variants, the epigenetic status of the genome (such as methylation ...

Answer

Microarray analysis of the 4282 nonmosaic samples identified all the aneuploidies and unbalanced rearrangements identified on karyotyping but did not identify balanced translocations and fetal triploidy.

Answer

Whole chromosome probes are particularly useful for examining chromosomal abnormalities, for example, when a piece of one chromosome is attached to the end of another chromosome. For many applications, FISH has largely been replaced by the use of microarrays. However, FISH remains useful for some tests.

Answer

To perform a microarray analysis, mRNA molecules are typically collected from both an experimental sample and a reference sample. For example, the reference sample could be collected from a healthy individual, and the experimental sample could be collected from an individual with a disease like cancer.

Answer

Microarray technology is finding its way into quantitative proteomics through the construction of what are most accurately called 'protein-detecting microarrays'1. A protein-detecting microarray comprises many different affinity reagents (frequently antibodies) arrayed at high spatial density on a solid support.

Answer

DNA microarray is an effective tool in transcriptomics that helps us in studying and analyzing the mRNA expression of almost every gene present in an organism. With the availability of whole-genome sequencing of microorganisms, it has now become possible to identify the genes with potential for bioremediation.

Answer

Microarray technology is a developing technology used to study the expression of many genes at once. It involves placing thousands of gene sequences in known locations on a glass slide called a gene chip. A sample containing DNA or RNA is placed in contact with the gene chip.

Answer

= Bioinformatics is a subdiscipline of biology and computer science concerned with the acquisition, storage, analysis, and dissemination of biological data, most often DNA and amino acid sequences.